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. 2013 Apr;93(4):521-8.
doi: 10.1189/jlb.0712328. Epub 2013 Jan 23.

Sphingosine-1-phosphate suppresses TLR-induced CXCL8 secretion from human T cells

Naveen Sharma  1 , Ajay Suresh Akhade, Ayub Qadri
Affiliations

Sphingosine-1-phosphate suppresses TLR-induced CXCL8 secretion from human T cells

Naveen Sharma et al. J Leukoc Biol. 2013 Apr.

Abstract

T cells produce a number of cytokines and chemokines upon stimulation with TLR agonists in the presence or absence of TCR signals. Here, we show that secretion of neutrophil chemoattractant CXCL8 from human T cell line Jurkat in response to stimulation with TLR agonists is reduced when cell stimulation is carried out in presence of serum. Serum does not, however, inhibit TCR-activated secretion of CXCL8 nor does it down-regulate TLR-costimulated IL-2 secretion from activated T cells. The molecule that can mimic the ability to bring about suppression in CXCL8 from TLR-activated T cells is serum-borne bioactive lipid, S1P. Serum and S1P-mediated inhibition require intracellular calcium. S1P also suppresses CXCL8 secretion from peripheral blood-derived human T cells activated ex vivo with various TLR ligands. Our findings reveal a previously unrecognized role for S1P in regulating TLR-induced CXCL8 secretion from human T cells.

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