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Second-order nonlinear optical microscopy of spider silk

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Abstract

Asymmetric \(\upbeta \)-sheet protein structures in spider silk should induce nonlinear optical interaction such as second harmonic generation (SHG) which is experimentally observed for a radial line and dragline spider silk using an imaging femtosecond laser SHG microscope. By comparing different spider silks, we found that the SHG signal correlates with the existence of the protein \(\upbeta \)-sheets. Measurements of the polarization dependence of SHG from the dragline indicated that the \(\upbeta \)-sheet has a nonlinear response depending on the direction of the incident electric field. We propose a model of what orientation the \(\upbeta \)-sheet takes in spider silk.

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Authors and Affiliations

  1. School of Materials Science, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, 923-1292, Japan

    Yue Zhao, Khuat Thi Thu Hien & Goro Mizutani

  2. School of Electronic and Computer Science, University of Southampton, Southampton, SO17 1BJ, UK

    Harvey N. Rutt

Authors
  1. Yue Zhao
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  2. Khuat Thi Thu Hien
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  3. Goro Mizutani
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  4. Harvey N. Rutt
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Correspondence to Goro Mizutani.

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Zhao, Y., Hien, K.T.T., Mizutani, G. et al. Second-order nonlinear optical microscopy of spider silk. Appl. Phys. B 123, 188 (2017). https://doi.org/10.1007/s00340-017-6766-z

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  • DOI: https://doi.org/10.1007/s00340-017-6766-z