Abstract
Cell to cell communication by biophotons has been demonstrated in plants, bacteria, animal neutrophil granulocytes and kidney cells. Whether such signal communication exists in neural cells is unclear. By developing a new biophoton detection method, called in situ biophoton autography (IBA), we have investigated biophotonic activities in rat spinal nerve roots in vitro. We found that different spectral light stimulation (infrared, red, yellow, blue, green and white) at one end of the spinal sensory or motor nerve roots resulted in a significant increase in the biophotonic activity at the other end. Such effects could be significantly inhibited by procaine (a regional anaesthetic for neural conduction block) or classic metabolic inhibitors, suggesting that light stimulation can generate biophotons that conduct along the neural fibers, probably as neural communication signals. The mechanism of biophotonic conduction along neural fibers may be mediated by protein-protein biophotonic interactions. This study may provide a better understanding of the fundamental mechanisms of neural communication, the functions of the nervous system, such as vision, learning and memory, as well as the mechanisms of human neurological diseases.
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References
R. P. Bajpai, P. K. Bajpai, D. Roy, Ultraweak photon emission in germinating seeds: a signal of biological order, J. Biolumin. Chemilumin., 1991, 6, 227–230.
Y. Isojima, T. Isoshima, K. Nagai, K. Kikuchi, H. Nakagawa, Ultraweak biochemiluminescence detected from rat hippocampal slices, NeuroReport, 1995, 6, 658–660.
B. Devaraj, M. Usa, H. Inaba, Biophotons: ultraweak light emission from living systems, Curr. Opin. Solid State Mater. Sci., 1997, 2, 88–193.
R. N. Tilbury, T. I. Cluickenden, Spectral and time dependence studies of the ultraweak bioluminescence emitted by the bacterium Escherichia coli, Photochem. Photobiol., 1988, 47, 145–150.
M. Kobayashi, M. Takeda, K. I. Ito, H. Kato, H. Inaba, Two dimensional photon counting imaging and spatiotemporal characterization of ultraweak photon emission from a rat’s brain in vivo, J. Neurosci. Methods, 1999, 93, 163–168.
S. Cohen, F.-A. Popp, Biophoton emission of human body, Indian J. Exp. Biol., 2003, 41, 440–445.
R. N. Tilbury, The effect of stress factors on the spontaneous photon emission from microorganisms, Experientia, 1992, 48, 1030–1041.
J. Slawinski, A. Ezzahir, M. Godlewski, T. Kwiecinska, Z. Rajfur, D. Sitko, D. Wierzuchowska, Stress-induced photon emission from perturbed organisms, Experientia, 1992, 48, 1041–1058.
H. J. Niggli, Artificial sunlight irradiation induces ultraweak photon emission in human skin fibroblasts, J. Photochem. Photobiol., B, 1993, 18, 281–285.
T. Amano, M. Kobayashi, B. Devaraj, M. Usa, H. Inaba, Ultraweak biophoton emission imaging of transplanted bladder cancer, Urol. Res., 1995, 23, 315–318.
Y. Kataoka, Y. Cui, A. Yamagata, M. Niigaki, T. Hirohata, N. Oishi, Y. Watanabe, Activity-dependent neural tissue oxidation emits intrinsic ultraweak photons, Biochem. Biophys. Res. Commun., 2001, 285, 1007–1011.
M. Nakano, Low-level chemiluminescence during lipid peroxidations and enzymatic reactions, J. Biolumin. Chemilumin., 1989, 4, 231–240.
Y. Z. Yoon, J. Kim, B. C. Lee, Y. U. Kim, S. K. Lee, K. S. Soh, Changes in ultraweak photon emission and heart rate variability of epinephrine-injected rats, Gen. Physiol. Biophys., 2005, 24, 147–159.
R.V. Wijk, E. P. Wijk, An introduction to human biophoton emission, Forsch. Komplementarmed. Klass. Naturheilkd., 2005, 12, 77–83.
R. Dobrin, C. Kirsch, S. Kirsch, J. Pierrakos, E. Schwartz, T. Wolff and Y. Zeira, Experimental measurements of the human energy field, in Psychoenergetic Systems: The Interface of Consciousness, Energy and Matter, ed. S. Krippner, Gordon and Breach, New York, 1979, 227–230.
T. J. Kim, Biophoton emission from fingernails and fingerprints of living human subjects, Acupunct. Electrother. Res., 2002, 27, 85–94.
I. Bókkon, Phosphene phenomenon: a new concept, BioSystems, 2008, 92, 168–174.
E. P. A. Van Wijk, J. Ackerman, R. Van Wijk, Effect of meditation on hand and forehead ultraweak photon emission, Forsch. Komplementärmed. Klass. Naturheilkd., 2005, 12, 107–112.
A. G. Gurwitsch, The mitogenetic radiation, Ann. Physol., 1934, 10.
F.-A. Popp, J. J. Chang, Q. Gu, and M. W. Ho, Nonsubstantial biocommunication in terms of Dicke’s theory, in Bioelectrodynamics and Biocommunication, ed. M.-W. Ho, F.-A. Popp and U. Warnke, World Scientific Publishing, Singapore, 1994, 293–317.
G. Albrecht-Buehler, Rudimentary form of cellular vision, Proc. Natl. Acad. Sci. U. S. A., 1992, 89, 8288–8292.
X. Shen, W. Mei, X. Xu, Activation of neutrophils by a chemically separated but optically coupled neutrophil population undergoing respiratory burst, Experientia, 1994, 50, 963–968.
C. Vidal-Valverde, New functional legume foods by germination: effect on the nutritive value of beans, lentils and peas, Eur. Food Res. Technol., 2002, 215, 472–477.
J. Dai, R. M. Buijs, W. Kamphorst, D. F. Swaab, Impaired axonal transport of cortical neurons in Alzheimer’s disease is associated with neuropathological changes, Brain Res., 2002, 948, 138–144.
M. Kobayashi, M. Takeda, T. Sato, Y. Yamazaki, K. Kaneko, K. Ito, H. Kato, H. Inaba, In vivo imaging of spontaneous ultraweak photon emission from a rat’s brain correlated with cerebral energy metabolism and oxidative stress, Neurosci. Res., 1999, 34, 103–113.
C. Tarba, C. A. Crăcium, Comparative study of the effects of procaine, lidocaine, tetracaine and dibucaine on the functions and ultrastructure of isolated rat liver mitochondria, Biochim. Biophys. Acta, Bioenerg., 1990, 1019, 19–28.
N. C. Shaner, P. A. Steinbach, R. Y. Tsien, A guide to choosing fluorescent proteins, Nat. Methods, 2005, 2, 905–909.
X. Shu, A. Royant, M. Z. Lin, T. A. Aguilera, V. Lev-Ram, P. A. Steinbach, R. Y. Tsien, Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome, Science, 2009, 324, 804–807.
R. K. Benninger, M. Hao, D. W. Piston, Multi-photon excitation imaging of dynamic processes in living cells and tissues, Rev. Physiol., Biochem., Pharmacol., 2008, 160, 71–92.
V. P. Skulachev, Mitochondrial filaments and clusters as intracellular power-transmitting cables, Trends Biochem. Sci., 2001, 26, 23–29.
M. Müller, S. L. Mironov, M. V. Ivannikov, J. Schmidt, D. W. Richter, Mitochondrial organization and motility probed by two-photon microscopy in cultured mouse brainstem neurons, Exp. Cell. Res., 2005, 303, 114–127.
V. N. Dedov, B. D. Roufogalis, Organisation of mitochondria in living sensory neurons, FEBS Lett., 1999, 456, 171–174.
R. Thar, M. Kühl, Propagation of electromagnetic radiation in mitochondria?, J. Theor. Biol., 2004, 230, 261–70.
V. V. Artem’ev, A. S. Goldobin, L. N. Gus’kov, Recording of light emission from a nerve, Biophysics, 1967, 12, 1111–1113.
F. A. Wininger, J. L. Schei, D. M. Rector, Complete optical neurophysiology: toward optical stimulation and recording of neural tissue, Appl. Opt., 2009, 48, D218–D224.
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Sun, Y., Wang, C. & Dai, J. Biophotons as neural communication signals demonstrated by in situ biophoton autography. Photochem Photobiol Sci 9, 315–322 (2010). https://doi.org/10.1039/b9pp00125e
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DOI: https://doi.org/10.1039/b9pp00125e
