In the present study, a minicolumn of sisal fiber loaded with alizarin fluorine blue is proposed as a preconcentration system for copper determination in tobacco leaf samples by flame atomic absorption spectrometry. During the optimization procedure, a two level full factorial design (2(4)) was used at the preliminary evaluation of four factors, involving the following variables: sampling flow rate, elution flow rate, buffer concentration and pH. Regarding the studied levels, this design has shown that buffer concentration and pH were significant factors. The experimental conditions established in the optimization step were: pH=4.75, buffer concentration of 0.005 mol L(-1) for elution with HCl 1.0 mol L(-1) this system allows the determination of copper content with a detection limit (LD) of 0.018 μg L(-1) and a quantification limit (LQ) of 0.061 μg L(-1) precision expressed as relative standard deviation (R.S.D.) of 4.65 and 5.07%, utilizing concentration of 10 and 2.0 μg L(-1), respectively, and a preconcentration factor of 75, for a sample volume of 50.0 mL. Accuracy was confirmed by copper determination in the standard reference material, NIST SRM 1570 a trace element units in Spinach Leaves and by spike tests with recovery levels ranging from 93 to 100%; the procedure was applied for copper determination in tobacco leaf samples collected in Cruz das Almas City, Bahia, Brazil. The achieved concentrations of the three samples analyzed varied from 0.15 to 0.52 μg g(-1).