102 captures
11 Jul 2004 - 23 Nov 2023
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Most Popular Articles...
Is it OK to use purified water instead of buffer to resuspend my oligos? I'm worried about the low pH of the water causing depurination of the DNA. Is this a legitimate concern?
modified: 7/1/2004
How do I anneal two oligos?
modified: 7/28/2004
I have been using your DNA oligos (~27-28bp, standard desalting for 25 nm) for PCR amplification of genes for cloning. I have recently discovered that two of the primers are incorrect after cloning and sequencing the PCR products. Is this a usual problem with the shorter oligos? PAGE purification is not an option for this scale of order. What do you suggest? Are the primers all bad, or just a small percentage truncated?
modified: 7/1/2004
I need a random 15-mer oligo for a pre-genomic amplification protocol. When I try to order it (NNNNNNNNNNNNNNN), you tell me that there is something wrong with it and you don't guarantee the product.
modified: 7/1/2004
Can you tell me the minimum number of bases that can be annealed?
modified: 7/1/2004
Latest Additions...
Do you synthesize 5 and 3-rhodamine oligos?
modified: 3/4/2005
What steps can I take to ensure the longest lifespan of an oligonucleotide possible? We suspect ours to be degrading faster than expected. We keep about 2-3 hundred ul (10uM) of working primers in the freezer and thaw before each use. Any suggestions?
modified: 3/4/2005
Hello, I want to design antisense RNA, clone it into a plasmid and transfect it into cells. Can you please help me with this. What type of purification should I use? What modifications do I need on the sense and antisense strand to ensure that the antisense oligo strand is transcribed in the vector. Should I include phosphorothioate modifications even though I will be annealing my oligos together? What are the advantages of having chimeric oligos with 2’-O-Methyl RNA bases flanking
modified: 3/2/2005
I have seen some recommendations for dual labeled flourescent probes to have the quencher placed on an internal nucleotide instead of at the 3 end (to lower background). Would you recommend this and do you perform this service?
modified: 3/2/2005
I found a tube for one of our oligonucleotide primers in the freezer. The problem is I cannot tell if it has already been resuspended or if it is just empty. There seems to be a film on the bottom of the tube, which makes me think it has not been resuspended. If I resuspend the “film,” is there a way to check to make sure that it was really the oligonucleotide on the bottom of the tube?
modified: 2/21/2005
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